THE DEVELOPMENT OF PLANT REGENERATION SYSTEMS FOR THE GENETIC IMPROVEMENT OF WALNUT Walt Tu1ecke and Gale McGranahan ABSTRACT
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چکیده
The techniques and capability to regenerate asexual embryos from walnut cotyledon tissue have been developed. Tissues from five Jug1ans regia cu1tivars, Jug1ans hindsii, and pterocarya sp. have been used. More than 100 asexual embryos have been selected from among the thousands produced. The sequence of procedures needed to obtain plants from these embryos have been worked out, and more than a dozen plants have been grown for 4 months in soil. These (and others) plants will be grown in the field and evaluated to determine whether they represent a clone or whether some are somac1ona1 variants. Similar procedures have been applied to 'Manregian' walnut endosperm tissues, and some asexual embryos have been obtained. No plants have yet been grown from these embryos.
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